Salary range: ≥ 25,000 and < 35,000€ annual gross
Employer: Electron Microscopy Platform of Universite de Picardie Jules Verne
Workplace: amiens – FRANCE
Skill area: Biology, Medicine, Health – Physiology and biology of organisms – Engineering
Application deadline: 5/15/2017
The project ENSEMBLE (2016-2019): In situ ENvironmental Scanning Electron Microscopy for Biological sampLEs is funded by Haut-de-France Region and results from a cooperation between the Plate-forme de Microscopie Electronique from Université de Picardie Jules Verne, two laboratories: Biologie des Plantes et Innovation (BIOPI EA 3900) and Unité de Génie Enzymatique et Cellulaire (GEC FRE CNRS 3580) and an industrial partner: Linea Semences de Lin. In this context, a postdoctoral position is proposed in the area of electron microscopy applied to bioresources.
Context and objectives:
In situ environmental scanning electron microscopy (ESEM) has shown promising results in observation of biological samples, however, this approach is still in a trial phase and its strong capabilities have hardly been exploited for the study of biological mechanisms. In situ ESEM will use as a novel approach for examination of plant tissues and their cell wall properties. More precisely, in situ ESEM approaches will be developed to examine the modification in morphology and mechanic aspects of plant cell walls and lignocellulosic biopolymers, as a result of seed mucilage extrusion, enzymatic modifications, or plant‐pathogen interactions. Micromechanical testing protocols will be implemented by targeting the general mechanisms of cell wall deformation. In the context of biorefinery, a microinjection device will be used to investigate ionic liquid pretreatment and enzymatic bioconversion of lignocellulosic biomass in situ. The findings of this project will shed light on both structure‐function relationship of cell wall constituents and their valorization.
The selected candidate will work in a professional and multidisciplinary environment associated to the technologically advanced equipment’s from the Plate-forme de Microscopie Electronique supervised by Professor Loic Dupont. More precisely, the candidate will develop innovative in situ approaches on an environmental scanning electron microscope FEI Quanta200F coupled to elemental analysis, micromanipulator with micro-indentor and micro-injector. Selected candidate will strongly interact with the two partner laboratories.
PhD in plant biology, biochemistry, green chemistry, chemistry or biotechnology.
Skills in electron microscopy (Environmental Scanning Electron Microscopy) will be a serious advantage.
Experience with in‐situ SEM Experience with Kleindiek Micromanipulator and associated accessories is a plus.
Team spirit, rigorous experimental working, inventiveness and sense of taking the lead
Language: French and/or English
Duration 2 years
Professeur Loic DUPONT
Directeur de la Plateforme de Microcopie Electronique de l’Université de Picardie Jules Verne
33 rue saint leu 80039 Amiens cedex