Biochips are commonly used in vitro for the multiplexed detection of biomarkers, as regards the in vivo molecular detection. No multiplexed approach and without labeling was developed to date. Still, such a tool could significantly advance diagnostic techniques as endoscopy effectively completing this imaging method. Indeed, the diagnosis would be facilitated by the detection, quantification and real-time location of specific biomarkers for certain diseases.
The objective of the project “MOLY ” is to design a “laboratory on fiber” which is capable of performing multiplexed bioassays, remote, in vivo, in real time and without marking. This unique tool will be dedicated, in the near future, to the medical diagnosis by endoscopy.
To achieve this goal, we propose to use assemblies of optical fibers nanostructured and functionalized. Fashioned in a relevant way and covered with a thin layer of gold or gold nanoparticles, these nano-structured optical fibers have interesting plasmonic properties. These optical properties will be exploited for remote biological analyzes. For this, an array of different biological probes will be carried on the nano-structured face of the fiber bundle to impart biological recognition properties. The functionalization with biomolecules can be done by conventional techniques using self-assembled thiol layers (Self-Assembled Monolayer SAM) or electrochemistry pyrrole or implement innovative methods (inkjet or microcontact , optical methods …). The network thus formed will be used in vivo for the specific detection, multiplexed and distance of biological targets.
The candidate ideally have physics and biology skills(physicist with experience in biological experimental experience or biologist introduced to instrumental development).
The post-doctoral wil be firstly charged of the development of the multi-fiber assemblies of biofunctionalization. He / She then characterize the sensitivities and detection limits in of the recognition of biological targets in solution, in tissues (models and real), and finally in vivo in mice. He / she will adapt the existing optical device for in vivo measurements. This project is highly interdisciplinary as it combines the optical skills, image processing, electrochemistry, surface chemistry, biochemistry, nano and micro-technologies applied to the field of biology. The candidate should have an interest in these scientific fields. In particular, he / she must have strong skills / knowledge in the field of optics. A biology experiment would be highly appreciated.
Domains: Physics – biologie – Medicine, health
Work place: Grenoble – France
Employer: University of Grenoble Alpes
Salaire range: 25 – 35k€/year
Application deadline : Feb, 28 2017
17, rue des martyrs, Grenoble, France